Figure 2
(a) An empty micro-patterned silicon chip designed for the Roadrunner II goniometer. The chip contains 12 µm sized pores that are spaced 90 and 100 µm apart, allowing well defined positioning of crystal-containing cells. (b) Sf9 insect cells containing in cellulo grown HEX-1 crystals were sucked into the chip pores. Visible intracellular crystals positioned in the pores are marked with yellow arrows; blue arrows point into empty pores. The images were recorded using reflected light on an Olympus SZX16 microscope. |