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Figure 5
Intrinsic fluorescence spectra and quenching profiles for EptA in different detergent micelles. (a) Fluorescence spectra and Stern–Volmer plots for native folded enzyme and (b) fluorescence spectra for the denatured enzyme. The emission wavelength maxima (λmax) for each condition are indicated above each curve. The red and blue coloured lines represent enzyme in DDM and DPC micelles, respectively. The solid lines represent EptAWT, the dashed lines represent EptATrp126/148Phe and the dotted lines represent EptATrp126/148/207Phe. Panels (i) and (iii) show the emission spectra of the enzymes and panels (ii) and (iv) in (a) show the Stern–Volmer plot for quenching using iodide. A linear regression was fitted using GraphPad prism with an R2 value above 0.95. Where a non-linear relationship was observed, only the initial linear slope was fitted.

Volume 8| Part 5| September 2021| Pages 732-746
ISSN: 2052-2525