Figure 2
Autocleavage of MtHigA2. (a) Size-exclusion chromatography of MtHigA2 before (top) and after (bottom) TEV cleavage. The major peak coloured yellow reduces substantially, and a small peak shown in green became the major peak after purification. (b) Schematic diagram of MtHigA2 and the corresponding SDS–PAGE. The colours correspond to the components in the MtHigA2 construct. |