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Figure 6
Genetic organization of the pamarR gene and EMSA of the PaMarR–DNA complex. (a) Genetic organization of the PaMarR and MMPL family transporter-encoding genes in the Paenisporosarcina sp. TG-14 genome and the upstream region sequence of pamarR. The PaMarR and MMPL family transporter genes are transcribed in the same direction. The putative −10 and −35 boxes of the pamarR promoter located in the intergenic region are marked in red. The putative PaMarR-binding sites with palindromes predicted by EMBOSS (http://emboss.bioinformatics.nl/cgi-bin/emboss/palindrome) are shown in red. (b) EMSA of PaMarR and DNA probes containing the putative PaMarR-binding sites in the intergenic region. The respective PaMarR protein samples with increasing concentrations (0, 0.3, 0.9, 1.5 and 2.1 µM) were incubated with oligonucleotide duplexes (0.5 µM) containing the putative binding sites 1 and 2. The PaMarR-free (F) and PaMarR-bound (B1, either putative binding site 1 or 2; B2, both putative binding sites 1 and 2) probes are indicated by arrows.

IUCrJ
Volume 8| Part 5| September 2021| Pages 842-852
ISSN: 2052-2525