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Figure 2
(a) The domains of the Mce SBPs. The Mce SBPs are characterized by having four domains referred to as the transmembrane (TM), MCE, helical and tail domains. The constructs of MtMce1A and MtMce4A used in this study are shown below in the same color coding. (b) Size-exclusion chromatography (SEC) elution profiles of MtMce1A38–325, MtMce1A126–454 and MtMce1A38–454 and of MtMce4A39–320, MtMce4A121–400 and MtMce4A36–400 on a 24 ml Superdex 200 10/300 column. The protein samples were analyzed by 12% SDS–PAGE (inset). (c) SEC elution profiles of MtMce1A36–148 and MtMce4A39–140 on a 120 ml Superdex 75 HiLoad 16/600 column. The protein samples were analyzed by 18% SDS–PAGE (inset). (d) CD spectra of MtMce1A38–325 (brown), MtMce1A126–454 (green), MtMce4A36–400 (black), MtMce4A39–320 (blue) and MtMce4A121–400 (pink). (e) CD spectra of MtMce1A38–454 (red), MtMce1A36–148 (yellow) and MtMce4A39–140 (maroon).

IUCrJ
Volume 8| Part 5| September 2021| Pages 757-774
ISSN: 2052-2525
https://doi.org/10.1107/S2052252521006199