Figure 5
Comparison of the steps to create binarized spot images from (a) an experimental image using (b), (c) the DL method and (e), (f) the standard routine. Furthermore, (d) and (g) are the results of arithmetic operations on the experimental and binary images: a − ac and a − af, respectively, showing the performance of the segmentations. Red arrows in (g) mark spots that are not segmented by the standard routine. |