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Figure 1
(a) and (b) Real space detector images and (c) and (d) corresponding diffraction mode detector images of a biomolecule-free 0.5% NaCl solution on a 400 mesh Quantifoil holey carbon grid with 2 µm holes plunge-cooled in boiling LN2. Both thick and thin ice were fully vitrified with no ice diffraction evident. The dashed lines in (c) and (d) indicate the expected positions of pure cubic ice diffraction (orange lines) at 1/d = 2.73, 4.45 and 5.22 nm−1 and of stacking disordered ice diffraction which, in addition to the peaks of cubic ice, typically has additional strong peaks at the hexagonal ice positions 2.57 and 2.91 nm−1 (yellow lines).

Volume 8| Part 6| November 2021| Pages 867-877
ISSN: 2052-2525