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Figure 5
Drift-corrected RMS particle displacement versus fluence, all measured using the same cryo-TEM. Grid type A is Quantifoil UltraAuFoil 1.2/1.3, Au foil with 1.2 µm holes on Au grids; type B is prototype Au foil with 1.2 µm holes on a Cu grid; type C is prototype Au foil with 1.2 µm holes on an Au grid. Samples 1–6 were cooled in boiling LN2, and sample 7 was cooled in ethane using a Vitrobot Mark IV. Samples 1 and 3 were partially vitrified; most holes gave good particle images but showed local ice, confirmed by appreciable intensity at ice ring positions in image FFTs. Samples 2 and 3–7 were largely vitrified with only a small minority of frames showing evidence of ice.

IUCrJ
Volume 8| Part 6| November 2021| Pages 867-877
ISSN: 2052-2525