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Figure 2
Dimeric structure of abMltA. (a) Multi-angle light-scattering (MALS) profile derived from the main size-exclusion chromatography (SEC) peak. The red line indicates the experimental molecular mass analyzed by MALS. (b) Crystallographic packing symmetry analysis. The two abMltA molecules found in the asymmetric unit are indicated by blue and light blue ribbon structures, while the other symmetric molecules are indicated by dark green and green ribbon structures. (c) Table summarizing the interaction details of the two types of interface analyzed by the PISA server. (d) Tentative dimeric structure of abMltA generated and analyzed by symmetry analysis and the PISA server. A magnified view of the region in the black box is provided to the right of the dimeric cartoon figure. Red and black dashed lines indicate salt bridges and hydrogen bonds, respectively. (e) Verification of the interfaces via mutagenesis. SEC profiles comparing the positions of eluted peaks between wild-type and mutant protein containing three mutated residues (Asp151 to Lys, Tyr162 to Trp and Glu164 to Lys). (f) MALS profiles of the triple mutant of abMltA. The red line indicates the experimental molecular mass analyzed by MALS.

IUCrJ
Volume 8| Part 6| November 2021| Pages 921-930
ISSN: 2052-2525
https://doi.org/10.1107/S2052252521008666