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Figure 7
BlaC–SUB complexes at Δtm = 66 ms. (a) Active site in subunit C with noncovalently bound intact sulbactam; left side, DEDiso map (contour: 3σ); right side, 2mFo − DFc map (contour: 1.7σ) after refinement. Close-by amino acids and the phosphate (Pi) are marked. (b) Active site in subunit B with trans-enamine bound to Ser70; left side, DEDiso map (contour: 3σ); right side, 2mFo − DFc map (contour: 1.7σ) after refinement. Red and blue dots show important differences between the subunits. Gln112 from the adjacent subunit is not located close by and Arg173 is extended in subunit B, leaving subunit B more accessible to ligands and substrate.

IUCrJ
Volume 8| Part 6| November 2021| Pages 878-895
ISSN: 2052-2525
https://doi.org/10.1107/S2052252521008125