Figure 6
(a) Projection of simulated rotavirus DLP, using PDB entry 3gzu, with the average peak SNRmf from 180 DLPs overlaid in white. (b) Example image from movie frame two, with only 1.5 e− Å−2 cumulative exposure. (c) Ratio of the rotationally averaged power spectrum of a simulation with solvent embedding to the base simulation as in (a). (d) Ratio of the rotationally averaged power spectrum of a simulation with solvent embedding and detector MTF to the previous simulation as in (c). (e) Ratio of the rotationally averaged power spectrum of a simulation with solvent embedding, detector MTF and residual intra-frame motion blur to the previous simulation as in (d). (f) Ratio of the rotationally averaged power spectrum of a simulation with solvent embedding, detector MTF, residual intra-frame motion blur and PDB B-factors to the previous simulation as in (e). Scale bar 500 Å. |