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Figure 4
Comparison of interfacial packing scores between atomic models derived using cryo-EM and crystallography for the same proteins. Each subplot in the figure corresponds to 1 of 3 clusters of identical two-protein sub-assemblies in cryo-EM and crystal structures. The distribution of packing scores of interface residues in constituent cryo-EM (green) and crystal (yellow) structures is represented as boxplots in each subplot. (a) Interface of α and β subunits in 20S proteasome structures from Thermoplasma acidophilum. (b) Interface of α and β subunits in hemoglobin structures from humans. (c) Interface of mitchondrial cysteine desulfurase and mitochondrial iron–sulfur cluster assembly enzyme in humans.

Volume 9| Part 1| January 2022| Pages 98-103
ISSN: 2052-2525