Figure 3
Data collection speeds using different image shift delay factors. (a) Rate of data collection using image shift delay factors of 0, 0.25, 0.5, 1 and 2 in super-resolution (SR) mode or hardware-binned (HB) mode. (b) Particle contribution of individual multishot positions contributing to the final 3D reconstruction for each of the recording conditions and image shift delay factor tested. The outlier in the hardware-binned image shift delay factor 0 is attributed to beam edge entering the field of the detector during data collection. For further breakdown of particle contributions see Fig S1. (c) Individual panels show visualization of coulombic potentials for amino acids Y29 and L115 for each of the eight apoferritin maps. |