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Figure 6
Examples of membrane protein crystallization modalities and structures solved. (a) Membrane protein structure determination in MicroED in a lipid and/or detergent environment mimicking the lipid bilayer. The structure of the NaK ion channel was determined from detergent micelles (Liu & Gonen, 2018BB66), mVDAC from lipid bicelles (Martynowycz et al., 2020BB72), the A2A-receptor from LCP crystals (Martynowycz, Shiriaeva et al., 2021BB73), whereas aqua­porin-0 (AQP0) was determined from double-layered crystals of the protein embedded in lipid bilayers using electron diffraction (Gonen et al., 2005BB40). (b) Top view showing the electron crystallography structure of AQP0 resolved lipid-protein packing interactions between the tetramer (yellow) and the surrounding lipid molecules (blue). (c) Top view showing the MicroED structure of mVDAC and the crystal packing of the monomers (yellow) where the map (blue) in between the barrels indicates the space is likely to be occupied by the lipid molecules.

IUCrJ
Volume 9| Part 2| March 2022| Pages 169-179
ISSN: 2052-2525
https://doi.org/10.1107/S2052252521013063