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Figure 2
ALD inhibition of 3′–5′ exonuclease activity. (a) MOPV NP-exo was incubated with HP4 dsRNA in the absence or presence of the catalytic ions Mg2+ or Mn2+ and a fixed concentration of ALD (100 µM). Reaction samples were taken after time intervals of 0, 5 and 30 min. The products were analyzed on urea–PAGE and visualized by autoradiography. (b) Inhibition of hTREX2 activity in the presence of 100 µM ALD. Left, inhibition of hTREX2 activity on single-stranded DNA (ssDNA); right, inhibition of hTREX2 activity using Y-shaped DNA with four-nucleotide 3′- and 5′-overhangs. (c) and (d) show representative gels showing the effect of ALD on the exonuclease activity of MOPV NP-exo and LCMV NP-exo, respectively. (e) and (f) are dose–response curves, from which IC50 values were calculated. For IC50 determination, the percentage of activity was measured by quantifying the total product formed after 5 min. IC50 values were then determined by fitting the dose–response curves. Reactions were performed in triplicate.

IUCrJ
Volume 9| Part 4| July 2022| Pages 468-479
ISSN: 2052-2525
https://doi.org/10.1107/S2052252522005061