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Figure 4
Real-world multi-grid screening with Smart Leginon Autoscreen followed by data collection with the Smart Leginon and the Ptolemy hole finder. Autoscreen readily allowed for the identification of (a) the best grid and (b) another decent grid. The Ptolemy hole finder from inside Smart Leginon was then used for high-quality data collection from (c) the best grid, leading to thousands of (d) exposure magnification images in (e) areas of moderately thick ice resulting in (f) relatively high-resolution CTF estimations, (g) high-quality 2D classes and a 3.1 Å structure (not shown).

IUCrJ
Volume 10| Part 1| January 2023| Pages 77-89
ISSN: 2052-2525
https://doi.org/10.1107/S2052252522010624