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Figure 5
Real-world Smart Leginon screening MSI grid analysis of a user grid. The MSI analysis, with square magnification images of several squares from Grid 3 in Fig. S15 in the first row, hole magnification images of corresponding holes in the second row, exposure magnification images from inside corresponding holes in the third row, and ice thickness estimations in the fourth row, allowed the operator and user to determine that medium-sized squares contain 30–40 nm-thick ice containing (d) non-overlapping particles of interest, while (a)–(c) squares with ice thinner than 30 nm ice were void of particles and (e) squares with ice on the order of 100 nm thick had overlapping particles. Scale bars are 10 µm for the first row, 5 µm for the second row and 100 nm for the third row.

IUCrJ
Volume 10| Part 1| January 2023| Pages 77-89
ISSN: 2052-2525
https://doi.org/10.1107/S2052252522010624