view article

Figure 1
Required target selection steps during single-particle cryo-EM data acquisition. (a) Low magnification overview (Atlas) of a cryo-EM grid. Human operator choices for squares that are suitable for imaging are marked with green boxes. Yellow and red boxes represent operator choices of squares with thick ice layers or broken support film, respectively. (b) Square with examples of operator choices of holes that are suitable for cryo-EM imaging marked with green circles. Yellow and red circles mark operator choices of holes that have suboptimal ice thickness or are covered by ice contamination, respectively. (c) High-magnification image of a hole with a thin ice layer. Areas for cryo-EM data acquisition are marked with blue boxes. (d) Cryo-EM image acquired under optimal conditions and in thin ice showing single particles of good density.

ISSN: 2052-2525