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Figure 4
Mutational analysis to confirm the dimerization interface of TLR15TIR. (a) Dimerization interface residues of TLR15TIR mutated to confirm that the BB loop, αC2 helix and DD loop are involved in TLR15TIR dimerization. The mutated residues (Ile753, Tyr779 and Phe813) are shown as green or orange spheres in the TLR15TIR dimer structure (light blue or gray ribbons) and are labeled with mutations (I753Y, Y779D and F813D). (b) Gel-filtration chromatography analysis of the dimerization-deficient TLR15TIR mutants in comparison with TLR15TIR. The SDS–PAGE analysis of the gel-filtration chromatography fractions 1–14 is shown in Fig. S5.

IUCrJ
Volume 10| Part 3| May 2023| Pages 352-362
ISSN: 2052-2525
https://doi.org/10.1107/S2052252523002956