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Figure 1
The FucO reaction and the compounds used. (a) FucO catalyzes the reversible aldehyde reduction/alcohol de­hydrogenase oxidation of short-chain aldehydes and their corresponding primary alcohols using NADH and NAD+ as cofactors, respectively. (b) Compounds studied as substrates and ligands. Compound 2 [(S)-lactaldehyde] is the physio­logical substrate. Compound 3 (phenyl­acetaldehyde) has been used in the laboratory-directed evolution experiment for screening the catalytic proficiency of variants. Compounds 4, 5 and 6 are more bulky substrates and compound 7 (3,4-di­meth­oxy­phenyl­acetamide) is a non-reactive substrate analog of 6. (c) Ordered sequential BiBi mechanism used as a model for the FucO-catalyzed aldehyde reduction.

IUCrJ
Volume 10| Part 4| July 2023| Pages 437-447
ISSN: 2052-2525
https://doi.org/10.1107/S205225252300444X