Figure 1
General mechanism of enolase in forward catalysis. (a) Opening and closing of the active site loops in enolase. The loop opening is thermodynamically more favorable in the PEP-bound than the 2PG-bound enzyme. Reprinted (adapted) with permission from Li & Hammes-Schiffer (2019). Copyright 2019 American Chemical Society. (b) General catalytic mechanism for forward catalysis. (c) Catalytic cycle for the forward reaction. |