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Figure 3
Structural comparison of the MtEno monomer at different stages of catalysis. (a) Cα superimposition of MtEno monomers solved in different catalytic stages: apo MtEno (gray), MtEno–2PG-canonical (brown), MtEno–2PG-alternate (pink) and MtEno–PEP (light blue) with loops 1, 2 and 3 highlighted. Focused sections of (b) loop 2 and (c) loop 3, with Cα displacement in MtEno–2PG-canonical when compared with the apo state, indicated by dashed lines. (d) CryoEM map curved around non-protein density at 7σ in which PEP and two Mg2+ ions fit in a similar orientation like that of 2PG-canonical and corresponding Mg2+ ions, shown in (f). (e) Hydrogen-bond-mediated interaction of PEP in canonical conformation within the binding pocket site. (f) |Fo| − |Fc| map showing electron density for 2PG-canonical at the 3σ level. (g) Hydrogen-bond-mediated interaction of 2PG-canonical. (h) |Fo| − |Fc| map showing the electron density for the 2PG-alternate at the 3σ level. (i) Hydrogen-bond-mediated interactions of the 2PG-alternate.

IUCrJ
Volume 10| Part 6| November 2023| Pages 738-753
ISSN: 2052-2525
https://doi.org/10.1107/S2052252523008485