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Figure 5
Mechanism of action and catalytic cycle for the reverse reaction. (a) Scheme of the proposed catalytic mechanism of the PEP to 2PG conversion. The reaction starts and completes one cycle in the apo state (top right). Hydrogen bonds are shown as yellow dashes. The position of MgB in the canonical 2PG state displaced in the alternate state is shown by a dotted circle. (b) Mechanism of reverse catalysis. Glu204 removes the proton from a water molecule to create a nucleophile (OH−) which attacks the sp2 carbonyl group. The intermediate carbanion is stabilized by resonance. Following the formation of the carbanion intermediate, Lys335 donates a proton to C3 to form 2PG

IUCrJ
Volume 10| Part 6| November 2023| Pages 738-753
ISSN: 2052-2525
https://doi.org/10.1107/S2052252523008485