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Figure 3
(a) FSCs of the extracted models and the reconstructed model with respect to the original model. (b) The original model of a yeast nucleolar pre-60S ribosomal subunit. (c), (d) and (e) show the extracted models corresponding to the top 11, 21 and 31 basis vectors with the largest norms, respectively. Their half-period resolutions are 3.32, 2.66 and 2.26 nm, respectively. (f) The model reconstructed from 10 000 simulated diffraction patterns by aligning the top 21 basis vectors. Its half-period resolution is 4.07 nm. (g) A low-resolution model obtained by applying a 4 nm FWHM Gaussian filter to the original model. In (b)–(g), the isosurfaces are plotted at 10% of the maximum density.

IUCrJ
Volume 11| Part 1| January 2024| Pages 92-108
ISSN: 2052-2525
https://doi.org/10.1107/S2052252523009831