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Figure 1
Purification and gel-filtration analysis of recombinant IMP. (a) SDS–PAGE of purified recombinant IMP on a 12% SDS–PAGE gel. The samples were loaded with 12 µl in each well. Lane M contains molecular-weight markers (labelled in kDa). (b) Gel-filtration standards for recombinant IMP. The calibration standard curve for size-exclusion chromatography was measured using the standard substances aprotinin (APR, 6.5 kDa), ribonuclease A (R, 13.7 kDa), carbonic anhydrase (CA, 29 kDa) and conalbumin (C, 75 kDa).

IUCrJ
Volume 11| Part 3| May 2024| Pages 384-394
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524003075