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Figure 5
(a) RT-PCR of Y. entomophaga culture at the late log phase, showing contemporaneous amplification of both yenB and yenC3 genes. Amplicons were visualized on a 1% agarose gel alongside the GeneRuler 1 kb DNA ladder (Thermo Scientific). (b) Multiple sequence alignment of YenC1, YenC2 and YenC3, highlighting the diagnostic sequences used to establish the presence of YenC3. (c) SDS–PAGE of purified Yen–Tc used to identify the constituent protein subunits. (d) Relative abundance of Yen–Tc component proteins, calculated using the summed intensities of unique peptides per protein.

IUCrJ
Volume 11| Part 3| May 2024| Pages 299-308
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524001969