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Figure 6
Influence of different numbers of diffraction patterns on the orientation determination and phase retrieval. Tests were conducted using the protein 6zfp. (a) Comparison of 3D Fourier volumes reconstructed by the PM-aided (blue) and random (magenta) methods using different numbers of diffraction patterns. All volumes are rendered at the same contour level. (b) Comparison of both methods using different numbers of patterns in terms of the CC shell. Solid lines represent the PM-aided method and dotted lines represent the random method. The horizontal line indicates the resolution of each reconstructed Fourier volume, where the CC shell is 0.5. (c) Comparison of the distribution of angular deviations using different numbers of diffraction patterns for the PM-aided method (blue) and the random method (magenta). The units for angular deviations are given in degrees. (d) Comparison of electron-density maps after iterative phasing of the Fourier volumes reconstructed by the PM-aided method under different numbers of patterns. In the upper row, the initial map is calculated with phases of the predicted model. In the lower row, the initial map is calculated with random phases. (e) Comparison of FSCs of the recovered electron-density maps using prior phases (solid lines) and random phases (dotted lines) under various numbers of patterns, plotted against the length of scattering vector. The horizontal line indicates the resolution of the recovered 3D electron-density map, where FSC drops to 0.5.

IUCrJ
Volume 11| Part 4| July 2024| Pages 602-619
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524004858