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Figure 7
Comparison of dark and active state models of CrPhotLOV1 with previously published structures. (a) Superposition of the dark-state model from Fedorov et al. (2003BB23) obtained at CT (magenta) with our dark state (gray) obtained at RT. (b) 2Fobslight(n) − Fcalc extrapolated electron density maps at Δt = 2.5 ms shown at 1.0 and 3.0σ (blue and orange mesh, respectively) around the Cys57–FMN and Gln120. (c) Superposition of the light-adapted state from Fedorov et al. (2003BB23) (yellow) with our 2.5 ms structure (purple) showing the translation of Gln120 and the difference in the geometry of the FMN–Cys57 adduct with the original 1n9l structure. Structural coordinates were superposed within Pymol with the cealign algorithm.

IUCrJ
Volume 11| Part 5| September 2024| Pages 792-808
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524005608