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Figure 4
(a) Evolutions of Rg, individual concentrations C of SERF1a and NT17, and molar ratio M, deduced from the SEC-SWAXS elution of the mixture. (b) The SWAXS data of the NT17–SERF1a complex (SASDVR5), merged from the data frames shadowed in (a), are fitted using Rosetta-fastsaxs with the model shown in (c). (c) The representative Rosetta-fastsaxs model with two NT17 (represented by a ribbon and green dots) binding to the N-terminal side of SERF1a (in red). (d) A zoom-in view for the binding of the N side of NT17 (colored coil) on the short helical segment (Site-1) and the long coil region (Site-2) on the N-terminal side of SERF1a (cf. Table 1[link]).

IUCrJ
Volume 11| Part 5| September 2024| Pages 849-858
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524006341