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Figure 1
Overview of a four-step workflow that makes use of the Roodmus framework. Nodes highlighted in yellow are performed using software external to the Roodmus framework; steps in blue use Roodmus utilities. (1) A user-provided MD simulation is used as the input to the data generation. (2) Time points from the trajectory may be sampled via Roodmus, which subsequently simulates micrographs using the Parakeet simulation package in which the conformation of each particle is sampled from these time points. (3) These synthetic micrographs can be processed using 3D reconstruction software, including HRAs. (4) Every step of the resulting reconstruction pipelines can then be analysed using Roodmus utilities to compare reconstruction outputs to ground truth information. Roodmus currently supports metadata from CryoSPARC and RELION pipelines as well as CryoDRGN and 3DFlex metadata.

IUCrJ
Volume 11| Part 6| November 2024| Pages 951-965
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524009321