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Figure 3
RD and fluence. (a) Power spectra of two micrographs simulated without (left) and with (right) RD. (b) FSC curves of reconstructions obtained with 8000 particles from datasets without (blue) and with (orange) RD. (c) ResLog analysis of datasets simulated without (blue) and with (orange) RD; the B factor from linear regression are shown. (d) 2D class averages from eight datasets with fluence ranging between 5 and 45 e− Å−2. The four best classes are shown along with their RELION class ranker score in yellow and the percentage of particles in each class in white. (e) Number of particles (left axis, solid line) and 2D classes which pass the threshold (right axis, dashed line) of 0.3 (black) or 0.5 (red) for the predicted class scores from RELION. (f) Resolution versus fluence plots using either 30 000 (red) or 8000 (blue) particles.

IUCrJ
Volume 11| Part 6| November 2024|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524009321