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Figure 3
Local 2DTM SNR and z-score maps of an apoferritin particle (a) and two tubulin patch particles (b, c). Each row shows the 3D template generated from the cisTEM program simulate (with similar views as the particles), the simulated particle at an underfocus of 500 nm, the 2DTM matched template, the SNR map and the z-score map centered on the particle. Targets that survived the cisTEM z-score threshold are circled. True positives (orange) are located near the center of the particles. Templates in rows (b) and (c) represent the tubulin patch viewed edge-on and from the side.

ISSN: 2052-2525