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Figure 8
Calculation of the 2DTM p-value and evaluation of three 2DTM metrics for a simulated clathrin montage. (a) An example of a clathrin montage segment with four particles arranged in a 2 × 2 grid. (b) Local maxima identified in the 2DTM z-score map of the montage segment in (a) using a 10-pixel radius. (c) A scatter plot of 2DTM SNR versus z-score, with true targets labeled in orange based on their expected location and orientation. In total, 92 of the 100 targets in the example montage were recovered as local maxima in the z-score map. (d) The quantile-normalized data, color-coded by their 2DTM p-values [−log(p-value)]. Data points not in the first quadrant are labeled in gray, as they are excluded from the p-value calculation. True targets are circled in orange. (e) A zoomed-in version of (d) showing the transformed z-score threshold. (f) ROC curves of the three 2DTM metrics, with shaded areas indicating the confidence intervals calculated from 2000 simulated particles.

IUCrJ
Volume 12| Part 2| March 2025|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524011771