view article
Figure 2
FLEXR-MSA reveals previously unnoticed isoform-specific conformations across all four 6DMP-bound human HSP90s. (a) Hsp90α (green), Hsp90β (blue), Grp94 (yellow) and Trap1 (red) bound to 6DMP (inset, PDB ligand ID 42C). (b) Ringer plot produced by FLEXR-MSA showing additional conformations (arrows) of a conserved binding-site Asp in Hsp90β chains A (∼340°) and D (∼190°). The solid line corresponds to 0σ. The gray dashed line corresponds to 0.3σ, the Ringer cutoff for modeling. The black dashed line corresponds to 1σ, the conventional modeling threshold.

IUCrJ
Volume 12| Part 2| March 2025| Pages 245-254
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525001332