view article
Figure 3
Homolog-specific HSP90 conformations. (a) Comparison of human (blue) and C. albicans (red) apo Hsp90α shows an additional conformation of a binding-site Met in the Ringer plot for the human protein. (b) Comparison of human and C. albicans Hsp90α bound to AUY-922 shows an additional conformation of a Ser in the C. albicans protein in the inset Ringer plot. (c) Radicicol (RDC) bound to C. albicans Hsp90α has an additional conformation of a conserved Asp in the C. albicans form, while Lys58/47 is more variable in the human form (d, e). (e) Comparison of SNX-2112 bound to human, C. albicans and T. brucei (purple) Hsp90α. The A and B chains are shown for human and C. albicans and chain A is shown for T. brucei. The inset Ringer plot shows that the Lys is conformationally variable between homologs bound to the conformation-responsive SNX-2112 ligand. Dotted lines represent hydrogen bonds detected with PyMOL. 2Fo − Fc maps are shown in blue and contoured at 1σ. Fo − Fc maps are shown in red and green and contoured at ±2σ.

IUCrJ
Volume 12| Part 2| March 2025| Pages 245-254
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525001332