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Figure 5
Impacts of BIR in MicroED data reduction. For (a) biotin and (c) Zn(II)-me­thio­nine crystals at 100 K, and (e) Co(II)-porphyrin crystals at room temperature all rotating at 0.09° s−1 during diffraction data acquisition: plots of the average change in mosaicity calculated with (light blue) and without (magenta) orientation refinements performed over all crystals of the type measured. For representative cases, (b), (d) and (f) show the diffraction frame acquired after delivery of 2.45 e− Å−2 fluence and rotation of 24.5°, superimposed first with labels of predicted reflection positions determined by XDS without orientation refinements (magenta), and then with those determined with orientation refinements implemented (teal). Labels show all peaks whose centroids were calculated to be ±1° of rotation from the displayed frame in each case.

IUCrJ
Volume 12| Part 2| March 2025|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252524012132