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Figure 2
Cryo-EM data for the DARPin–apoferritin scaffold (model 6c) bound to GFP. (a) The 2D class averages display well aligned 24-mer cores along with clear density for the fused 17 kDa DARPin and the attached 27 kDa GFP. (b) A composite cryo-EM map, following 3D refinements, reveals the GFP attached to a rigid imaging scaffold. (c) The corresponding gold-standard Fourier shell correlation (FSC) curves are presented, achieving a resolution of 2.97 Å based on a correlation threshold of 0.143.

IUCrJ
Volume 12| Part 3| May 2025| Pages 393-402
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525003021