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Figure 3
Near-atomic resolution map of the cargo protein GFP. (a) Two side views of the local resolution density map, showing bound GFP at a resolution of 3.47 Å, are presented, with each view rotated 180° relative to the other. This map was generated following multi-body refinement using RELION 3.1.2. (b) A ribbon diagram illustrates the GFP structure, where the helix (Trp57–Tyr92) along the central axis of the β-barrel (β1–β11) is interrupted by the chromophore (CRO) depicted as green sticks. (c) Focused views of the density map cover all GFP β-strands and the GFP chromophore, including its surrounding amino acids.

IUCrJ
Volume 12| Part 3| May 2025| Pages 393-402
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525003021