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Figure 4
Cryo-EM data of the DARPin–apoferritin scaffold (model 4) complexed with MBP. (a) The consensus cryo-EM map, resulting from 3D refinements, reveals MBP attached to a stabilized imaging scaffold. (b) The local resolution map distinctly presents the apoferritin core at atomic resolutions close to 2.3 Å and the MBP C-terminal domain (CTD) with a near-atomic resolution of approximately 3.8 Å. Furthermore, the resolutions for MBP N-terminal domain (NTD) regions away from the DARPin exceed 4 Å. (c) The corresponding gold-standard Fourier shell correlation (FSC) curves are presented, achieving a resolution of 2.69 Å based on a correlation threshold of 0.143. (d) The density fits of DARPin and its bound MBP in two different views, which are related by a 90° rotation, are highlighted.

IUCrJ
Volume 12| Part 3| May 2025| Pages 393-402
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525003021