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Figure 2
Plunge-through-film ligand-solution deposition mechanism suitable for achieving few-millisecond time points. (a) Roughly 2 µl of ligand solution is loaded into a 2 mm diameter loop. The loop is formed from two interlocking halves attached to hinged arms, which are held together by opposing magnets in each arm. The arms project downwards from the platform that attaches to the deposition vertical translation stage (Fig. 1[link] and Supplementary Fig. S6) and fit within the plunge bore. When the platform is moved to its lowest position, the loop is positioned ∼2 mm above the LN2 surface, corresponding to a nominal time point of 1 ms. (b) Liquid is transferred onto the samples and support as they pass through the ligand-solution film spanning the loop. Glancing impact of the gonio­meter base with the hinged arms forces them apart. With the arms out of the way, the sample, base and wand continue to the LN2.

IUCrJ
Volume 12| Part 3| May 2025|
ISSN: 2052-2525
https://doi.org/10.1107/S205225252500288X