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Figure 2
Improved ice distribution of mixed samples using MIU. (a, b) Representative atlas (left), square (center) and high-magnification images of apoferritin (right) obtained with BIU (a) and MIU (b), demonstrating the ice and particle distributions achieved with each method. A dark blue circle indicates regions of thick ice, and a light blue circle indicates regions of usable squares for targeting on the leftmost panel of (a). (c) The atlas (left), representative micrograph (right) and representative 2D class averages obtained from the preparation of mixed apoferritin and aldolase samples using MIU. (d) EM maps of aldolase (top, ∼2.9 Å) and apoferritin (bottom, ∼2.5 Å), with representative peptide density shown beneath each map.

IUCrJ
Volume 12| Part 6| November 2025| Pages 710-724
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525008838