Figure 6
Reconstruction of a tile from a focal series of PtIr with padding. (a) Amplitude and (b) phase images of a reconstruction of a tile of a focal series padded to twice the size corresponding to the same region as in Fig. 4 (a). The yellow outline delimits the part extracted from the original micrograph series before padding. At a defocus of −1660 Å at 200 kV and for the main reflection at 2.25 Å, the average delocalization is ∼18 Å. (c) Amplitude and (d) phase images of the reconstruction where the right half of the frequency-space representation was eliminated to isolate a side band. The yellow outline denotes the original images before padding, with the delocalized signal recreated to the left of the edge. The reconstruction spills over the edge on the right because of the spread of low-frequency information from the conjugate Ewald sphere, resulting in the blurred appearance. Scale bar: 20 Å. |