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Figure 1
Anti-rhinoviral efficacy and enzymatic inhibition of AG7404. (A) Dose-response curve analysis of AG7404 against hRV-B14, -A16 and -A21. HeLa cells were mock-infected (blue triangle) or infected with hRV-B14 (red circle), hRV-A16 (yellow square) and hRV-A21 (green rhombus) with various concentrations of AG7404. After 72 h, cell viability was measured using the MTT assay. Data represent means (±SD) from at least two independent experiments performed in duplicate. (B) Inhibitory activity of AG7404 against purified hRV-B14 3C protease. The enzymatic assay was performed using a colorimetric peptide substrate (EALFQ-pNA), and the release of pNA was measured at 405 nm to calculate the IC50 value. Data represent means (±SD) from triplicate experiments.

IUCrJ
Volume 13| Part 1| January 2026| Pages 19-30
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525008929