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Figure 2
How X-ray dose and crystal size affect achievable resolution for still-image SSX datasets of modelled spherical insulin crystals in a tophat beam of the same size. Six crystal and beam sizes were modelled: 100, 50, 25, 10, 5 and 1 µm, using four different X-ray photon fluxes: (a) 1 × 1011, (b) 1 × 1012, (c) 1 × 1013 and (d) 1 × 1015 photons s−1. The 1 × 1015 photons s−1 were modelled with an energy bandwidth of 0.4%, mimicking a DMM pink beam. All other fluxes were assumed to be monochromatic at 0.01%. The x axes of these plots are given in ADER, as calculated by RADDOSE-3D (Zeldin et al., 2013aView full citation; Zeldin et al., 2013bView full citation) with the resolution at CC1/2 = 0.3 on the y axes. The quadrant of each plot where the resolution and dose are below 2 Å and 150 kGy, respectively, is highlighted with a green rectangle. Complete data were modelled but not all datasets could be successfully processed and hence do not appear on the plots.

IUCrJ
Volume 13| Part 2| March 2026| Pages 184-197
ISSN: 2052-2525
https://doi.org/10.1107/S2052252525011224