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Figure 2
Purification and crystallization of CreD using buffer A. (a) Representative SDS–PAGE showing CreD in buffer A. (b) Trigonal CreD crystal. Scale bar: 0.100 mm. (c) 2mFo − DFc electron-density maps of subunits A and F of the trigonal CreD crystal are shown in light blue and light orange, respectively. The density was contoured at 1σ. A model of the orthorhombic CreD crystal is superimposed. Subunits A (x, y, z) and C (x, −y + 1, −z + 2) of the orthorhombic CreD crystal model are shown in light green and turquoise, respectively. Parts of subunits A and C of the orthorhombic CreD crystal model that are missing in the trigonal CreD crystal are shown in blue and pink, respectively.

IUCrJ
Volume 13| Part 2| March 2026| Pages 169-183
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526000072