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Figure 3
Thermal stability versus pH, ionic strength and additives for CreD: buffer C [20 mM HEPES, 20%(v/v) glycerol pH 6.8] yielded the highest thermal stability. Left: thermal denaturation profiles showing normalized fluorescence as a function of temperature. Right: ThermoFluor-derived Tm for CreD varying (a) pH in the presence of 150 mM NaCl and 10%(v/v) glycerol, (b) NaCl concentration in the presence of 20 mM Tris–HCl pH 7.5, 10%(v/v) glycerol and (c) glycerol, DTT and NaNO3 in the presence of 20 mM HEPES pH 6.8. Buffer A [20 mM HEPES, 20%(v/v) glycerol pH 8.0] was used as the control to assess stability (gray line). The standard deviation of Tm for CreD is indicated by vertical lines in the right panels [visible only for HEPES pH 7.5 in (a) and b5, b7, b12 and buffer A in (c)].

IUCrJ
Volume 13| Part 2| March 2026| Pages 169-183
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526000072