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Figure 4
Purification and crystallization of CreD using buffer C. (a) SDS–PAGE showing CreD in buffer C. (b) Orthorhombic CreD crystals. Scale bar: 0.100 mm. (c) Electron-density maps of the orthorhombic CreD crystal. 2mFo − DFc electron-density maps of subunits A and C are shown in light blue and light orange, respectively. The density is contoured at 1σ. (d) Resolution of native data for trigonal and orthorhombic crystals collected at a wavelength of 1.0000 Å. The resolution was determined using the XDS and XSCALE programs (Kabsch, 2010View full citation). The turquoise squares represent the mean resolution of CreD crystals. The significance of the difference between the two groups was examined by a two-tailed Wilcoxon rank-sum test: P = 0.0082. (e) Solvent content for the native data, calculated from the unit cell (Matthews, 1968View full citation). The turquoise squares represent the mean solvent content of CreD crystals. The significance of the difference between the two groups was examined by a two-tailed Wilcoxon rank-sum test: P = 0.0040.

IUCrJ
Volume 13| Part 2| March 2026| Pages 169-183
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526000072