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Figure 7
Thermal stability versus pH, ionic strength and additives for HIRA(644–1017): buffer H [20 mM HEPES–NaOH pH 6.8, 10%(v/v) glycerol, 500 mM NaCl, 2 mM DTT] yielded the highest thermal stability. Variation of (a) pH values in the presence of 10%(v/v) glycerol and 150 mM NaCl, (b) NaCl concentration in the presence of 20 mM Tris–HCl pH 7.5, (c) NaNO3 or MgCl2 in the presence of 20 mM Tris–HCl pH 7.5, 10%(v/v) glycerol, 300 mM NaCl, 2 mM DTT and (d) DTT and glycerol concentrations in the presence of 20 mM HEPES–NaOH pH 6.8 and 500 mM NaCl. Buffer G [20 mM HEPES–NaOH pH 7.5, 10%(v/v) glycerol, 300 mM NaCl, 2 mM DTT] was used as the control to assess stability (gray line). The standard deviation of Tm is indicated by vertical lines in the right panels [visible only for 2 mM DTT and buffer G in (d)].

IUCrJ
Volume 13| Part 2| March 2026| Pages 169-183
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526000072