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Figure 8
HIRA(644–1017) precipitated after one day of tag-cleavage reaction in buffer D [20 mM Tris–HCl pH 8.5, 10%(v/v) glycerol, 50 mM DTT, 300 mM NaCl] and was soluble in buffer E [20 mM Tris–HCl pH 8.5, 10%(v/v) glycerol, 50 mM DTT, 500 mM NaCl] for three days.

IUCrJ
Volume 13| Part 2| March 2026| Pages 169-183
ISSN: 2052-2525