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Figure 9
Application of DSF to improve the diffraction quality of HIRA(644–1017) crystals. (a) Photographs of representative crystals. Needle cluster crystals appeared under condition No. 14 of the Stura FootPrint screen. Thin plate crystals formed after seeding with CS-A (0.60 M ammonium sulfate, 0.15 M sodium citrate pH 5.5) or CS-B (0.40 M ammonium sulfate, 0.15 M sodium citrate pH 5.5), but did not appear after seeding with CS-C (0.60 M ammonium sulfate, 0.15 M MES–NaOH pH 6.5). The thick plate crystal was obtained in the presence of YCl3, and crystals resembling thin plates were obtained in the presence of ethyl acetate or acetone. Scale bar: 0.100 mm. (b) ThermoFluor-derived Tm for HIRA(644–1017) with variation in pH, precipitant concentration and additives. CS-B was used as the control to assess stability (blue line). The standard deviation of Tm is indicated by vertical lines (visible only for CS3, CS4, CS8, CS22, CS42, CS43, CS56, CS57, CS59 and CS62). Details of the additives are provided in Supplementary Table S2. (c) Resolution of the native SAD, native and Se-SAD data collected at wavelengths of 2.7000, 0.9790–1.0000 and 0.9784–0.9790 Å, respectively. The resolution was determined using method 2 in the DISTL program (Zhang et al., 2006View full citation). The turquoise squares indicate the mean resolution. The significance of the difference between the two groups was examined by a two-tailed Wilcoxon rank-sum test: P = 0.0077 for native and P = 0.0003 for Se-SAD.

IUCrJ
Volume 13| Part 2| March 2026| Pages 169-183
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526000072